PH-Sensitive Chitosan Nanoparticles Charged With Dolutegravir As Milk And Food Admixture For Paediatric Anti-HIV Therapy
The present study aims to develop Chitosan-free-based polymeric nanoparticles of anti-HIV drug Dolutegravir, to aid appropriate dose adjustment and ease of oral administration as milk and food admixture for children. The isolated Chitosan from the crab shell mintages Portunus Sanguinolentus has been characterized for their physicochemical props. Nanoparticles were evolved with motleying ratio of drug: Chitosan and taxed for particle size (140-548 nm), zeta potential (+26 mV) with a maximum of 75 % drug content. Nanoparticles displayed improved stability and drug release in the 0 N HCl medium equated to pure drug. The MTT assay and the Syncytia inhibition assay in C8166 (T-lymphatic cell line) infected with HIV(IIIB) viral strain, which showed better therapeutic efficiency and lesser cytotoxicity compared to the pure drug. In consonance with the data geted, the use of chitosan from a novel source for drug delivery carrier has gived exceptional panoramas for deporting drugs efficiently to paediatrics.
Monitoring freshness of chicken breast by utilizing natural halochromic curcumin adulterated chitosan/PEO nanofibers as an intelligent package.Intelligent packaging is important to get information about real time quality of foods. Healthcare of this study was to develop an electrospun nanofiber halochromic pH sensor film practicing curcumin, chitosan (CS) and polyethylene oxide (PEO) to monitor chicken freshness. Conductivity and rheological behavior of CS/PEO/curcumin answers were mensurated to understand the effect of solution attributes on the morphology of the characters. The morphological characteristics of nanofiber films were investigated by Field Emission Scanning Electron Microscopy (FESEM). Average diameter of the characters was detected to be between 283 ± 27 nm and 338 ± 35 nm. It was concluded that increasing CS amount in nanofibers lessened the diameter of the roughages.
Thermal analysis and water vapor permeability features of the pH sensor were also examined. Color modifications of curcumin laded CS/PEO nanofiber film was appraised on chicken breast package at 4 °C. The color of nanofiber film switched from bright yellow to reddish color which provided an opportunity to detect color changes by even the naked eyes of the untrained consumer. As a quality indicator, surface pH changes of the chicken breast and TVB-N (total volatile basic nitrogen) were measured. At the end of the day 5, pH value of 6 ±0 and TVB-N concentration of 23 ±3 mg/100 g indicated that food was at the edge of the acceptance level. As a result, curcumin charged nanofiber filled the expectation and gave an opportunity to visualize real time monitoring of chicken spoilage.Shelf-life extension of pomegranate arils utilizing chitosan nanoparticles loaded with Satureja hortensis essential oil.
BACKGROUND: This study was directed in two offices to improve the antimicrobial activity and stability of Satureja hortensis essential oil (SEO) and its encroachments on the quality of pomegranate arils. In Seebio Dietary Supplements , SEO was capsulized by an ionic gelation technique into 142-267 nm chitosan nanoparticles (CSNPs). In the second part of the experiment, the CSNPs and CSNPs-SEO were applied to improve storability of pomegranate arils. Arils were dipped in water (control), CSNPs and CSNPs-SEO for 5 min. After superficial water removal, arils were jamed into polystyrene boxes and stored at 5 °C for 18 days finded on spectrophotometry analysis, the encapsulation efficiency (EE) of SEO-stretched CSNPs (CSNPs-SEO) diminished from 26% to 7% and their loading capacity (LC) increased from 4% to 6%, respectively, upon increasing the initial SEO content from 0 to 0 g g(-1) of chitosan. Phytochemicals and water content were maintained, and microbial enumerations were abbreviated in the coated arils during storage. Total phenol and antioxidant activity minifyed during storage.
At the end of storage, the highest total phenol content (2980 mg gallic acid equivalents L(-1) ) was found in arils treated with CSNPs-SEO, whereas ascorbic acid content was maximal (6 mg L(-1) ) in arils treated with CSNPs.